Electroporation of transgenic DNAs in the sea squirt Ciona

Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The electroporation method described here is probably the mainstay of sea squirt (Ciona) research, because the utility of transgene expression in staged embryo populations enables a wide array of biological questions to be addressed. It allows rapid identification and characterization of cis-regulatory DNA, such as tissue-specific enhancers. Electroporation of plasmids expressing fluorescent reporter genes permits live imaging and lineage tracing. Finally, structure-function relationships can be examined by expressing dominant-negative or constitutively active forms of specific proteins using appropriate cell-specific enhancers.

Original languageEnglish (US)
JournalCold Spring Harbor Protocols
Volume4
Issue number12
DOIs
StatePublished - Dec 22 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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