Electrodeless dielectrophoresis of single- and double-stranded DNA

Chia Fu Chou, Jonas O. Tegenfeldt, Olgica Bakajin, Shirley S. Chan, Edward C. Cox, Nicholas Darnton, Thomas Duke, Robert H. Austin

Research output: Contribution to journalArticlepeer-review

387 Scopus citations


Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.

Original languageEnglish (US)
Pages (from-to)2170-2179
Number of pages10
JournalBiophysical Journal
Issue number4
StatePublished - Oct 1 2002

All Science Journal Classification (ASJC) codes

  • Biophysics


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