Electrodeless dielectrophoresis of single- and double-stranded DNA

Chia Fu Chou; Jonas O. Tegenfeldt; Olgica Bakajin; Shirley S. Chan; Edward C. Cox; Nicholas Darnton; Thomas Duke; Robert H. Austin

doi:10.1016/S0006-3495(02)73977-5

Electrodeless dielectrophoresis of single- and double-stranded DNA

Chia Fu Chou, Jonas O. Tegenfeldt, Olgica Bakajin, Shirley S. Chan, Edward C. Cox, Nicholas Darnton, Thomas Duke, Robert H. Austin

Research output: Contribution to journal › Article › peer-review

392 Scopus citations

Abstract

Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.

Original language	English (US)
Pages (from-to)	2170-2179
Number of pages	10
Journal	Biophysical Journal
Volume	83
Issue number	4
DOIs	https://doi.org/10.1016/S0006-3495(02)73977-5
State	Published - Oct 1 2002

All Science Journal Classification (ASJC) codes

Biophysics

Access to Document

10.1016/S0006-3495(02)73977-5

Cite this

@article{4f93c60618a84a108c51cac55a8b8ff3,

title = "Electrodeless dielectrophoresis of single- and double-stranded DNA",

abstract = "Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.",

author = "Chou, {Chia Fu} and Tegenfeldt, {Jonas O.} and Olgica Bakajin and Chan, {Shirley S.} and Cox, {Edward C.} and Nicholas Darnton and Thomas Duke and Austin, {Robert H.}",

note = "Funding Information: This work was supported by National Institutes of Health Grants HG01506 and GM55453, the Royal Society, and New Jersey Science and Technology Council Grant E08580.",

year = "2002",

month = oct,

day = "1",

doi = "10.1016/S0006-3495(02)73977-5",

language = "English (US)",

volume = "83",

pages = "2170--2179",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - Electrodeless dielectrophoresis of single- and double-stranded DNA

AU - Chou, Chia Fu

AU - Tegenfeldt, Jonas O.

AU - Bakajin, Olgica

AU - Chan, Shirley S.

AU - Cox, Edward C.

AU - Darnton, Nicholas

AU - Duke, Thomas

AU - Austin, Robert H.

N1 - Funding Information: This work was supported by National Institutes of Health Grants HG01506 and GM55453, the Royal Society, and New Jersey Science and Technology Council Grant E08580.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.

AB - Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.

UR - http://www.scopus.com/inward/record.url?scp=0036789421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036789421&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(02)73977-5

DO - 10.1016/S0006-3495(02)73977-5

M3 - Article

C2 - 12324434

AN - SCOPUS:0036789421

SN - 0006-3495

VL - 83

SP - 2170

EP - 2179

JO - Biophysical Journal

JF - Biophysical Journal

IS - 4

ER -

Electrodeless dielectrophoresis of single- and double-stranded DNA

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this