TY - JOUR
T1 - Electrode colonization by the Feammox bacterium Acidimicrobiaceae sp. strain A6
AU - Ruiz-Urigüen, Melany
AU - Shuai, Weitao
AU - Jaffé, Peter R.
N1 - Funding Information:
Funding for the sediment field and laboratory work of this research was provided by the Program of International S&T Cooperation, National Key Research and Development Program of China (grant 2016YFE0106600). Funding for MECs was provided by Princeton University's Andlinger Center for Energy and the Environment and by the Princeton Environmental Institute. The Instituto de Fomento al Talento Humano of Ecuador and the Mary and Randall Hack '69 research grant provided funding for M.R.-U. We thank Bruce Logan for providing the first MEC prototypes and Shan Huang for maintaining and providing pure A6 culture. We thank Claire White and Nishant Garg for their collaboration in soil surface area analysis. We also thank Florence Ling from Princeton University for her assistance in using the environmental scanning electron microscope
Publisher Copyright:
© 2018 American Society for Microbiology.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Acidimicrobiaceae sp. strain A6 (A6), from the Actinobacteria phylum, was recently identified as a microorganism that can carry out anaerobic ammonium (NH 4 + ) oxidation coupled to iron reduction, a process also known as Feammox. Being an iron-reducing bacterium, A6 was studied as a potential electrode-reducing bacterium that may transfer electrons extracellularly onto electrodes while gaining energy from NH 4 + oxidation. Actinobacteria species have been overlooked as electrogenic bacteria, and the importance of lithoautotrophic iron reducers as electrodereducing bacteria at anodes has not been addressed. By installing electrodes in the soil of a forested riparian wetland where A6 thrives, in soil columns in the laboratory, and in A6-bioaugmented constructed wetland (CW) mesocosms and by operating microbial electrolysis cells (MECs) with pure A6 culture, the characteristics and performances of this organism as an electrode-reducing bacterium candidate were investigated. In this study, we show that Acidimicrobiaceae sp. strain A6, a lithoautotrophic bacterium, is capable of colonizing electrodes under controlled conditions. In addition, A6 appears to be an electrode-reducing bacterium, since current production was boosted shortly after the CWs were seeded with enrichment A6 culture and current production was detected in MECs operated with pure A6, with the anode as the sole electron acceptor and NH 4 + as the sole electron donor.
AB - Acidimicrobiaceae sp. strain A6 (A6), from the Actinobacteria phylum, was recently identified as a microorganism that can carry out anaerobic ammonium (NH 4 + ) oxidation coupled to iron reduction, a process also known as Feammox. Being an iron-reducing bacterium, A6 was studied as a potential electrode-reducing bacterium that may transfer electrons extracellularly onto electrodes while gaining energy from NH 4 + oxidation. Actinobacteria species have been overlooked as electrogenic bacteria, and the importance of lithoautotrophic iron reducers as electrodereducing bacteria at anodes has not been addressed. By installing electrodes in the soil of a forested riparian wetland where A6 thrives, in soil columns in the laboratory, and in A6-bioaugmented constructed wetland (CW) mesocosms and by operating microbial electrolysis cells (MECs) with pure A6 culture, the characteristics and performances of this organism as an electrode-reducing bacterium candidate were investigated. In this study, we show that Acidimicrobiaceae sp. strain A6, a lithoautotrophic bacterium, is capable of colonizing electrodes under controlled conditions. In addition, A6 appears to be an electrode-reducing bacterium, since current production was boosted shortly after the CWs were seeded with enrichment A6 culture and current production was detected in MECs operated with pure A6, with the anode as the sole electron acceptor and NH 4 + as the sole electron donor.
KW - Acidimicrobiaceae sp. A6
KW - Actinobacteria
KW - Ammonium oxidation
KW - Anaerobic
KW - Electrode-reducing bacteria
KW - Feammox
KW - Iron reduction
KW - Lithoautotrophic
KW - Wetland soils
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U2 - 10.1128/AEM.02029-18
DO - 10.1128/AEM.02029-18
M3 - Article
C2 - 30291122
AN - SCOPUS:85058514745
SN - 0099-2240
VL - 84
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 24
M1 - e02029-18
ER -