Electric birefringence measurements of native, denatured, and renatured xanthan

Gregory J. Besio; Ian M. Leavesley; Robert K. Prud'Homme; Raymond Farinato

doi:10.1002/app.1987.070330312

Electric birefringence measurements of native, denatured, and renatured xanthan

Gregory J. Besio, Ian M. Leavesley, Robert K. Prud'Homme, Raymond Farinato

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17 Scopus citations

Abstract

Electric birefringence measurements were performed on three xanthan solutions to determine conformational differences between native xanthan, denatured xanthan (heated in 4 M urea to 90°C and cooled), and renatured xanthan (heated and cooled). The birefringence of samples exposed to pulses of 1000 V for 1 ms were analyzed to determine longest relaxation times and steady‐state birefringence. The results showed the following order for steady state birefringence, native > renatured > denatured, and longest relaxation time, renatured > native > denatured. The results are interpreted in terms of helix reformation and aggregation upon renaturing.

Original language	English (US)
Pages (from-to)	825-834
Number of pages	10
Journal	Journal of Applied Polymer Science
Volume	33
Issue number	3
DOIs	https://doi.org/10.1002/app.1987.070330312
State	Published - Feb 20 1987

All Science Journal Classification (ASJC) codes

General Chemistry
Surfaces, Coatings and Films
Polymers and Plastics
Materials Chemistry

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10.1002/app.1987.070330312

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title = "Electric birefringence measurements of native, denatured, and renatured xanthan",

abstract = "Electric birefringence measurements were performed on three xanthan solutions to determine conformational differences between native xanthan, denatured xanthan (heated in 4 M urea to 90°C and cooled), and renatured xanthan (heated and cooled). The birefringence of samples exposed to pulses of 1000 V for 1 ms were analyzed to determine longest relaxation times and steady‐state birefringence. The results showed the following order for steady state birefringence, native > renatured > denatured, and longest relaxation time, renatured > native > denatured. The results are interpreted in terms of helix reformation and aggregation upon renaturing.",

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AU - Besio, Gregory J.

AU - Leavesley, Ian M.

AU - Prud'Homme, Robert K.

AU - Farinato, Raymond

PY - 1987/2/20

Y1 - 1987/2/20

N2 - Electric birefringence measurements were performed on three xanthan solutions to determine conformational differences between native xanthan, denatured xanthan (heated in 4 M urea to 90°C and cooled), and renatured xanthan (heated and cooled). The birefringence of samples exposed to pulses of 1000 V for 1 ms were analyzed to determine longest relaxation times and steady‐state birefringence. The results showed the following order for steady state birefringence, native > renatured > denatured, and longest relaxation time, renatured > native > denatured. The results are interpreted in terms of helix reformation and aggregation upon renaturing.

AB - Electric birefringence measurements were performed on three xanthan solutions to determine conformational differences between native xanthan, denatured xanthan (heated in 4 M urea to 90°C and cooled), and renatured xanthan (heated and cooled). The birefringence of samples exposed to pulses of 1000 V for 1 ms were analyzed to determine longest relaxation times and steady‐state birefringence. The results showed the following order for steady state birefringence, native > renatured > denatured, and longest relaxation time, renatured > native > denatured. The results are interpreted in terms of helix reformation and aggregation upon renaturing.

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Electric birefringence measurements of native, denatured, and renatured xanthan

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