EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λgtWES system

P. Leder, D. Tiemeier, Lynn William Enquist

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

A derivative of bacteriophage λ has been modified and tested together with an appropriate host system to meet the criteria of EK2 biologic containment for cloning DNA from higher organisms. In this report certain of the safety features are summarized and some of the tests carried out to confirm the containment properties of the vector are described. The cloning efficiency of this system, together with available gene purification and hybrid screening technology, indicate that it can be used to clone DNA fragments carrying specific, unique mammalian genes.

Original languageEnglish (US)
Pages (from-to)175-177
Number of pages3
JournalScience
Volume196
Issue number4286
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

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