EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λgtWES system

P. Leder; D. Tiemeier; L. Enquist

doi:10.1126/science.322278

EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λgtWES system

P. Leder, D. Tiemeier, L. Enquist

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Abstract

A derivative of bacteriophage λ has been modified and tested together with an appropriate host system to meet the criteria of EK2 biologic containment for cloning DNA from higher organisms. In this report certain of the safety features are summarized and some of the tests carried out to confirm the containment properties of the vector are described. The cloning efficiency of this system, together with available gene purification and hybrid screening technology, indicate that it can be used to clone DNA fragments carrying specific, unique mammalian genes.

Original language	English (US)
Pages (from-to)	175-177
Number of pages	3
Journal	Science
Volume	196
Issue number	4286
DOIs	https://doi.org/10.1126/science.322278
State	Published - 1977

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abstract = "A derivative of bacteriophage λ has been modified and tested together with an appropriate host system to meet the criteria of EK2 biologic containment for cloning DNA from higher organisms. In this report certain of the safety features are summarized and some of the tests carried out to confirm the containment properties of the vector are described. The cloning efficiency of this system, together with available gene purification and hybrid screening technology, indicate that it can be used to clone DNA fragments carrying specific, unique mammalian genes.",

author = "P. Leder and D. Tiemeier and L. Enquist",

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AU - Tiemeier, D.

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EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λgtWES system

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