Efficient prime editing in two-cell mouse embryos using PEmbryo

Rebecca P. Kim-Yip, Ryan McNulty, Bradley Joyce, Antonio Mollica, Peter J. Chen, Purnima Ravisankar, Benjamin K. Law, David R. Liu, Jared E. Toettcher, Evgueni A. Ivakine, Eszter Posfai, Britt Adamson

Research output: Contribution to journalArticlepeer-review

Abstract

Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice.

Original languageEnglish (US)
JournalNature biotechnology
DOIs
StateAccepted/In press - 2024

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology
  • Biomedical Engineering

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