Effects of microcompartmentation on flux distribution and metabolic pools in chlamydomonas reinhardtii chloroplasts

Anika Küken, Frederik Sommer, Liliya Yaneva-Roder, Luke C.M. Mackinder, Melanie Höhne, Stefan Geimer, Martin C. Jonikas, Michael Schroda, Mark Stitt, Zoran Nikoloski, Tabea Mettler-Altmann

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.

Original languageEnglish (US)
Article numbere37960
JournaleLife
Volume7
DOIs
StatePublished - Oct 2018

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Effects of microcompartmentation on flux distribution and metabolic pools in chlamydomonas reinhardtii chloroplasts'. Together they form a unique fingerprint.

  • Cite this

    Küken, A., Sommer, F., Yaneva-Roder, L., Mackinder, L. C. M., Höhne, M., Geimer, S., Jonikas, M. C., Schroda, M., Stitt, M., Nikoloski, Z., & Mettler-Altmann, T. (2018). Effects of microcompartmentation on flux distribution and metabolic pools in chlamydomonas reinhardtii chloroplasts. eLife, 7, [e37960]. https://doi.org/10.7554/eLife.37960