TY - JOUR
T1 - Effects of microcompartmentation on flux distribution and metabolic pools in chlamydomonas reinhardtii chloroplasts
AU - Küken, Anika
AU - Sommer, Frederik
AU - Yaneva-Roder, Liliya
AU - Mackinder, Luke C.M.
AU - Höhne, Melanie
AU - Geimer, Stefan
AU - Jonikas, Martin C.
AU - Schroda, Michael
AU - Stitt, Mark
AU - Nikoloski, Zoran
AU - Mettler-Altmann, Tabea
N1 - Funding Information:
We thank Carola Päpke and Joost van Dongen for the usage of equipment for photosynthesis rate measurements and Marc-Aurel Schöttler and Arren Bar-Even for critical reading. AK acknowledges support by the Max Planck Society and TM-A acknowledges support by the Deutsche Forschungsge-meinschaft (EXC 1028). FS, MSt, MSch, and TM-A acknowledge support by the Federal Ministry of Education and Research (BMBF), Germany, within the frame of the GoFORSYS Research Unit for Systems Biology (FKZ0313924). MJ acknowledges support by the National Science Foundation (EF-1105617), the National Institutes of Health (DP2-GM-119137), and the Simons Foundation and HHMI (55108535).
Publisher Copyright:
© Küken et al.
PY - 2018/10
Y1 - 2018/10
N2 - Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.
AB - Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.
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U2 - 10.7554/eLife.37960
DO - 10.7554/eLife.37960
M3 - Article
C2 - 30306890
AN - SCOPUS:85056562033
SN - 2050-084X
VL - 7
JO - eLife
JF - eLife
M1 - e37960
ER -