Effects of block copolymer properties on nanocarrier protection from in vivo clearance

Suzanne M. D'Addio, Walid Saad, Steven M. Ansell, John J. Squiers, Douglas H. Adamson, Margarita Herrera-Alonso, Adam R. Wohl, Thomas R. Hoye, Christopher W. MacOsko, Lawrence D. Mayer, Christine Vauthier, Robert K. Prud'homme

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Drug nanocarrier clearance by the immune system must be minimized to achieve targeted delivery to pathological tissues. There is considerable interest in finding in vitro tests that can predict in vivo clearance outcomes. In this work, we produce nanocarriers with dense PEG layers resulting from block copolymer-directed assembly during rapid precipitation. Nanocarriers are formed using block copolymers with hydrophobic blocks of polystyrene (PS), poly-ε-caprolactone (PCL), poly-d,l-lactide (PLA), or poly-lactide-co- glycolide (PLGA), and hydrophilic blocks of polyethylene glycol (PEG) with molecular weights from 1 kg/mol to 9 kg/mol. Nanocarriers with paclitaxel prodrugs are evaluated in vivo in Foxn1nu mice to determine relative rates of clearance. The amount of nanocarrier in circulation after 4 h varies from 10% to 85% of initial dose, depending on the block copolymer. In vitro complement activation assays are conducted to correlate in vivo circulation to the protection of the nanocarrier surface from complement binding and activation. Guidelines for optimizing block copolymer structure to maximize circulation of nanocarriers formed by rapid precipitation and directed assembly are proposed, relating to the relative sizes of the hydrophilic and hydrophobic blocks, the hydrophobicity of the anchoring block, the absolute size of the PEG block, and polymer crystallinity. The in vitro results distinguish between the poorly circulating PEG5 k-PCL9 k and the better circulating nanocarriers, but could not rank the better circulating nanocarriers in order of circulation time. Analysis of PEG surface packing on monodisperse 200 nm latex spheres indicates that the size of the hydrophobic PCL, PS, and PLA blocks are correlated with the PEG blob size. Suggestions for next steps for in vitro measurements are made.

Original languageEnglish (US)
Pages (from-to)208-217
Number of pages10
JournalJournal of Controlled Release
Volume162
Issue number1
DOIs
StatePublished - Aug 20 2012

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Keywords

  • Circulation
  • Complement activation
  • Nanoparticle
  • Polycaprolactone
  • Polyethylene glycol
  • Polylactic acid

Fingerprint Dive into the research topics of 'Effects of block copolymer properties on nanocarrier protection from in vivo clearance'. Together they form a unique fingerprint.

  • Cite this

    D'Addio, S. M., Saad, W., Ansell, S. M., Squiers, J. J., Adamson, D. H., Herrera-Alonso, M., Wohl, A. R., Hoye, T. R., MacOsko, C. W., Mayer, L. D., Vauthier, C., & Prud'homme, R. K. (2012). Effects of block copolymer properties on nanocarrier protection from in vivo clearance. Journal of Controlled Release, 162(1), 208-217. https://doi.org/10.1016/j.jconrel.2012.06.020