TY - JOUR
T1 - Effect of double-stranded RNAs from sindbis virus-infected cells on rabbit reticulocyte and chick embryo cell-free protein synthesis
AU - Shenk, Thomas E.
AU - Stollar, Victor
PY - 1972/12/22
Y1 - 1972/12/22
N2 - 1. Cell-free polypeptide synthesis in rabbit reticulocyte lysates was inhibited by extracts of Sindbis virus-infected cells and double-stranded RNAs which accumulate in Sindbis-infected cells. 2. All species of double-stranded RNA isolated from Sindbis-infected cells were found to have the same inhibitory ability, and caused significant inhibition at concentrations as low as 1 · 10-10-1 · 10-11 g/ml. 3. Cell-free polypeptide synthesis in chick embryo lysates was insensitive to Sindbis-infected cell extracts, double-stranded RNA isolated from Sindbis-infected cells, and a synthetic double-stranded polymer, poly(rI)-poly(rC), at all concentrations tested. The chick embryo cell-free extracts appeared to initiate polypeptide synthesis in vitro, since incorporation of 3H-labeled amino acids into acid-precipitable polypeptides by these extracts was inhibited by aurintricarboxylic acid (8 · 10-5 M) and pactamycin (5 · 10-7 and 10-6 M). 4. Poly(U)-directed reticulocyte and chick embryo lysates at high Mg2+ concentrations were insensitive to inhibition by double-stranded RNA at all concentrations tested.
AB - 1. Cell-free polypeptide synthesis in rabbit reticulocyte lysates was inhibited by extracts of Sindbis virus-infected cells and double-stranded RNAs which accumulate in Sindbis-infected cells. 2. All species of double-stranded RNA isolated from Sindbis-infected cells were found to have the same inhibitory ability, and caused significant inhibition at concentrations as low as 1 · 10-10-1 · 10-11 g/ml. 3. Cell-free polypeptide synthesis in chick embryo lysates was insensitive to Sindbis-infected cell extracts, double-stranded RNA isolated from Sindbis-infected cells, and a synthetic double-stranded polymer, poly(rI)-poly(rC), at all concentrations tested. The chick embryo cell-free extracts appeared to initiate polypeptide synthesis in vitro, since incorporation of 3H-labeled amino acids into acid-precipitable polypeptides by these extracts was inhibited by aurintricarboxylic acid (8 · 10-5 M) and pactamycin (5 · 10-7 and 10-6 M). 4. Poly(U)-directed reticulocyte and chick embryo lysates at high Mg2+ concentrations were insensitive to inhibition by double-stranded RNA at all concentrations tested.
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U2 - 10.1016/0005-2787(72)90295-X
DO - 10.1016/0005-2787(72)90295-X
M3 - Article
C2 - 4641255
AN - SCOPUS:0015520316
SN - 0005-2787
VL - 287
SP - 501
EP - 513
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 3
ER -