E2-C, a cyclin-selective ubiquitin carrier protein required for the destruction of mitotic cyclins

Alexander Aristarkhov, Esther Eytan, Ameena Moghe, Arie Admon, Avram Hershko, Joan V. Ruderman

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

Ubiquitin-dependent proteolysis of the mitotic cyclins A and B is required for the completion of mitosis and entry into the next cell cycle. This process is catalyzed by the cyclosome, an ≃22S particle that contains a cyclin-selective ubiquitin ligase activity, E3-C, that requires a cyclin- selective ubiquitin carrier protein (UBC) E2-C. Here we report the purification and cloning of E2-C from clam oocytes. The deduced amino acid sequence of E2-C indicates that it is a new UBC family member. Bacterially expressed recombinant E2-C is active in in vitro cyclin ubiquitination assays, where it exhibits the same substrate specificities seen with native E2-C. These results demonstrate that E2-C is not a homolog of UBC4 or UBC9, proteins previously suggested to be involved in cyclin ubiquitination, but is a new UBC family member with unique properties.

Original languageEnglish (US)
Pages (from-to)4294-4299
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number9
DOIs
StatePublished - Apr 30 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'E2-C, a cyclin-selective ubiquitin carrier protein required for the destruction of mitotic cyclins'. Together they form a unique fingerprint.

Cite this