Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices

Nikolce Gjorevski, Alexandra S. Piotrowski, Victor D. Varner, Celeste M. Nelson

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


Collective cell migration drives tissue remodeling during development, wound repair, and metastatic invasion. The physical mechanisms by which cells move cohesively through dense three-dimensional (3D) extracellular matrix (ECM) remain incompletely understood. Here, we show directly that migration of multicellular cohorts through collagenous matrices occurs via a dynamic pulling mechanism, the nature of which had only been inferred previously in 3D. Tensile forces increase at the invasive front of cohorts, serving a physical, propelling role as well as a regulatory one by conditioning the cells and matrix for further extension. These forces elicit mechanosensitive signaling within the leading edge and align the ECM, creating microtracks conducive to further migration. Moreover, cell movements are highly correlated and in phase with ECM deformations. Migrating cohorts use spatially localized, long-range forces and consequent matrix alignment to navigate through the ECM. These results suggest biophysical forces are critical for 3D collective migration.

Original languageEnglish (US)
Article number11458
JournalScientific reports
Issue number1
StatePublished - 2015

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices'. Together they form a unique fingerprint.

Cite this