Abstract
A proteomics approach was used to study the drug-induced phospholipids in rat hepatocytes. Rat hepatocytes were isolated by collagenase perfusion and cultured as monolayers. The appearance of phospholipids in the treated samples was confirmed by light and electron microscopy. The proteins with drug-induced expression level changes were subjected to in-gel enzymatic digestion and analyzed by mass spectrometry (MALDI-MS). The results show an increase in the number of proteins resolved on 2D gels and a corresponding improved detection of changes in expression levels.
| Original language | English (US) |
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| Pages | 259-260 |
| Number of pages | 2 |
| State | Published - 2002 |
| Externally published | Yes |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 6/2/02 → 6/6/02 |
All Science Journal Classification (ASJC) codes
- Spectroscopy