Drosophila exocyst components sec5, sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane

Johanna Langevin; Matthew J. Morgan; Carine Rossé; Victor Racine; Jean Baptiste Sibarita; Sandra Aresta; Mala Murthy; Thomas Schwarz; Jacques Camonis; Yohanns Bellaïche

doi:10.1016/j.devcel.2005.07.013

Drosophila exocyst components sec5, sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane

Johanna Langevin
, Matthew J. Morgan
, Carine Rossé
, Victor Racine
, Jean Baptiste Sibarita
, Sandra Aresta
, Mala Murthy
, Thomas Schwarz
, Jacques Camonis
, Yohanns Bellaïche

Research output: Contribution to journal › Article › peer-review

230 Scopus citations

Abstract

The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.

Original language	English (US)
Pages (from-to)	365-376
Number of pages	12
Journal	Developmental cell
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/j.devcel.2005.07.013
State	Published - Sep 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2005.07.013

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@article{9e9c35853bfa440ba8155396ea84eb22,

title = "Drosophila exocyst components sec5, sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane",

abstract = "The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.",

author = "Johanna Langevin and Morgan, \{Matthew J.\} and Carine Ross{\'e} and Victor Racine and Sibarita, \{Jean Baptiste\} and Sandra Aresta and Mala Murthy and Thomas Schwarz and Jacques Camonis and Yohanns Bella{\"i}che",

note = "Funding Information: Y.B. and J.L. thank F. Schweisguth (Ecole Normale Sup{\'e}rieure, UMR 8544, Paris, France) for support at the beginning of this project. We thank K. Basler, H. Bellen, P. Bryant, H. Oda, R. Cohen, G. Gonzales, D. Ready, J. Sisson, W. Sullivan, A. Wodarz, A. Zahraoui, the Developmental Studies Hybridoma Bank, and the Bloomington Stock Center for strains and antibodies. We thank J. Salamero and V. Fraisier for advice including confocal microscopy. The exocyst two-hybrid screen would not have been possible without the contribution of P. Chavrier (Institut Curie). Y.B. thanks A. Morineau for encouragement and support. We thank N. David, A. Echard, R. Le Borgne, and J. Young for critical reading of the manuscript. This work was supported by grants from the Association pour la Recherche sur le Cancer (ARC 4726 and ARC 7744 to Y.B. and ARC 5440 to J.C.), the F{\'e}d{\'e}ration pour la Recherche M{\'e}dicale, the CNRS, the Curie Institute, grants from the Ministry of Research (ACI program grants), and GenHomme Network Grant (02490-6088) to Hybrigenics and Institut Curie. ",

year = "2005",

month = sep,

doi = "10.1016/j.devcel.2005.07.013",

language = "English (US)",

volume = "9",

pages = "365--376",

journal = "Developmental cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Drosophila exocyst components sec5, sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane

AU - Langevin, Johanna

AU - Morgan, Matthew J.

AU - Rossé, Carine

AU - Racine, Victor

AU - Sibarita, Jean Baptiste

AU - Aresta, Sandra

AU - Murthy, Mala

AU - Schwarz, Thomas

AU - Camonis, Jacques

AU - Bellaïche, Yohanns

N1 - Funding Information: Y.B. and J.L. thank F. Schweisguth (Ecole Normale Supérieure, UMR 8544, Paris, France) for support at the beginning of this project. We thank K. Basler, H. Bellen, P. Bryant, H. Oda, R. Cohen, G. Gonzales, D. Ready, J. Sisson, W. Sullivan, A. Wodarz, A. Zahraoui, the Developmental Studies Hybridoma Bank, and the Bloomington Stock Center for strains and antibodies. We thank J. Salamero and V. Fraisier for advice including confocal microscopy. The exocyst two-hybrid screen would not have been possible without the contribution of P. Chavrier (Institut Curie). Y.B. thanks A. Morineau for encouragement and support. We thank N. David, A. Echard, R. Le Borgne, and J. Young for critical reading of the manuscript. This work was supported by grants from the Association pour la Recherche sur le Cancer (ARC 4726 and ARC 7744 to Y.B. and ARC 5440 to J.C.), the Fédération pour la Recherche Médicale, the CNRS, the Curie Institute, grants from the Ministry of Research (ACI program grants), and GenHomme Network Grant (02490-6088) to Hybrigenics and Institut Curie.

PY - 2005/9

Y1 - 2005/9

N2 - The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.

AB - The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.

UR - https://www.scopus.com/pages/publications/24144477936

UR - https://www.scopus.com/pages/publications/24144477936#tab=citedBy

U2 - 10.1016/j.devcel.2005.07.013

DO - 10.1016/j.devcel.2005.07.013

M3 - Article

C2 - 16224820

AN - SCOPUS:24144477936

SN - 1534-5807

VL - 9

SP - 365

EP - 376

JO - Developmental cell

JF - Developmental cell

IS - 3

ER -

Drosophila exocyst components sec5, sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane

Abstract

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