Drosophila argonaute-2 is required early in embryogenesis for the assembly of centric/centromeric heterochromatin, nuclear division, nuclear migration, and germ-cell formation

Girish Deshpande, Gretchen Calhoun, Paul Schedl

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

The RNA-induced silencing complex (RISC) or the RISC complex mediates RNAi and is comprised of proteins belonging to the dicer and Argonaute family proteins. Here we show that Argonaute-2 (ago-2) is required for proper nuclear migration, pole cell formation, and cellularization during the early stages of embryonic development in Drosophila. We have traced these defects back to the nuclear division cycles. Unlike wild type, nuclear division is asynchronous in ago-2 embryos and there are defects in chromosome condensation, nuclear kinesis, and assembly of spindle apparatus. The aberrations in the nuclear division cycle are correlated with defects in the formation of centric/centromeric heterochromatin and point to a failure in the assembly of functional centromeres.

Original languageEnglish (US)
Pages (from-to)1680-1685
Number of pages6
JournalGenes and Development
Volume19
Issue number14
DOIs
StatePublished - Jul 2005

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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