DNA transformation via local heat shock

Sha Li, L. Meadow Anderson, Jui Ming Yang, Liwei Lin, Haw Yang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

This work describes transformation of foreign DNA into bacterial host cells by local heat shock using a microfluidic system with on-chip, built-in platinum heaters. Plasmid DNA encoding ampicillin resistance and a fluorescent protein can be effectively transformed into the DH5α chemically competent E. coli using this device. Results further demonstrate that only one-thousandth of volume is required to obtain transformation efficiencies as good as or better than conventional practices. As such, this work complements other lab-on-a-chip technologies for potential gene cloning/therapy and protein expression applications.

Original languageEnglish (US)
Article number013902
JournalApplied Physics Letters
Volume91
Issue number1
DOIs
StatePublished - Nov 19 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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