DNA transformation by local heat shock

Sha Li; L. Meadow Anderson; Liwei Lin; Haw Yang

DNA transformation by local heat shock

Sha Li, L. Meadow Anderson, Liwei Lin, Haw Yang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

DNA transformation by local heat shock using a MEMS device has been successfully demonstrated using an on-chip micro heater and corresponding microfluidic system. The transformation experiments have been conducted and 200% improvement in transformation efficiency was shown as compared with control tests using conventional methodology. Moreover, the green fluorescent proteins expressed from the transformed DNA in cells were verified under a fluorescence microscope, showing all cells grown from antibiotic selection after transformation have transformed DNA working correctly as expected. As such, this work complements other on-going lab-on-a-chip researches for possible gene cloning, gene therapy, and protein expression applications.

Original language	English (US)
Title of host publication	Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages	533-536
Number of pages	4
State	Published - 2007
Externally published	Yes
Event	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan Duration: Jan 21 2007 → Jan 25 2007

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Country/Territory	Japan
City	Kobe
Period	1/21/07 → 1/25/07

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Cite this

@inproceedings{ca66ae2ed9d549eaa24e173ac02f5bf0,

title = "DNA transformation by local heat shock",

abstract = "DNA transformation by local heat shock using a MEMS device has been successfully demonstrated using an on-chip micro heater and corresponding microfluidic system. The transformation experiments have been conducted and 200% improvement in transformation efficiency was shown as compared with control tests using conventional methodology. Moreover, the green fluorescent proteins expressed from the transformed DNA in cells were verified under a fluorescence microscope, showing all cells grown from antibiotic selection after transformation have transformed DNA working correctly as expected. As such, this work complements other on-going lab-on-a-chip researches for possible gene cloning, gene therapy, and protein expression applications.",

author = "Sha Li and Anderson, {L. Meadow} and Liwei Lin and Haw Yang",

year = "2007",

language = "English (US)",

isbn = "1424409519",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "533--536",

booktitle = "Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007",

note = "20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 ; Conference date: 21-01-2007 Through 25-01-2007",

}

Li, S, Anderson, LM, Lin, L & Yang, H 2007, DNA transformation by local heat shock. in Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007., 4433152, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 533-536, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 1/21/07.

DNA transformation by local heat shock. / Li, Sha; Anderson, L. Meadow; Lin, Liwei et al.
Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 533-536 4433152 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - DNA transformation by local heat shock

AU - Li, Sha

AU - Anderson, L. Meadow

AU - Lin, Liwei

AU - Yang, Haw

PY - 2007

Y1 - 2007

N2 - DNA transformation by local heat shock using a MEMS device has been successfully demonstrated using an on-chip micro heater and corresponding microfluidic system. The transformation experiments have been conducted and 200% improvement in transformation efficiency was shown as compared with control tests using conventional methodology. Moreover, the green fluorescent proteins expressed from the transformed DNA in cells were verified under a fluorescence microscope, showing all cells grown from antibiotic selection after transformation have transformed DNA working correctly as expected. As such, this work complements other on-going lab-on-a-chip researches for possible gene cloning, gene therapy, and protein expression applications.

AB - DNA transformation by local heat shock using a MEMS device has been successfully demonstrated using an on-chip micro heater and corresponding microfluidic system. The transformation experiments have been conducted and 200% improvement in transformation efficiency was shown as compared with control tests using conventional methodology. Moreover, the green fluorescent proteins expressed from the transformed DNA in cells were verified under a fluorescence microscope, showing all cells grown from antibiotic selection after transformation have transformed DNA working correctly as expected. As such, this work complements other on-going lab-on-a-chip researches for possible gene cloning, gene therapy, and protein expression applications.

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M3 - Conference contribution

AN - SCOPUS:52249123653

SN - 1424409519

SN - 9781424409518

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 533

EP - 536

BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007

T2 - 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007

Y2 - 21 January 2007 through 25 January 2007

ER -

DNA transformation by local heat shock

Abstract

Publication series

Other

All Science Journal Classification (ASJC) codes

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