DNA transformation by local heat shock

Sha Li, L. Meadow Anderson, Liwei Lin, Haw Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

DNA transformation by local heat shock using a MEMS device has been successfully demonstrated using an on-chip micro heater and corresponding microfluidic system. The transformation experiments have been conducted and 200% improvement in transformation efficiency was shown as compared with control tests using conventional methodology. Moreover, the green fluorescent proteins expressed from the transformed DNA in cells were verified under a fluorescence microscope, showing all cells grown from antibiotic selection after transformation have transformed DNA working correctly as expected. As such, this work complements other on-going lab-on-a-chip researches for possible gene cloning, gene therapy, and protein expression applications.

Original languageEnglish (US)
Title of host publicationProceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages533-536
Number of pages4
StatePublished - Dec 1 2007
Externally publishedYes
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: Jan 21 2007Jan 25 2007

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period1/21/071/25/07

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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    Li, S., Anderson, L. M., Lin, L., & Yang, H. (2007). DNA transformation by local heat shock. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 533-536). [4433152] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).