TY - JOUR
T1 - Dna-Protein Interactions at Telomeres in Ciliated Protozoans
AU - Gottschling, Daniel E.
AU - Zakian, Virginia A.
N1 - Funding Information:
Work in our laboratory is supported by grants from the National Institutes of Health and the American Cancer Society. D.E.G. is supported by an N.I.H.p ostdoctoral fellowship. We thank A. Pluta, K. Runge, and R. Wellinger for their critical comments on the paper, J. Bryant for typing the manuscript, and C. Price and T. Cech for communicating results prior to publication.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - This chapter discusses the DNA–protein complexes found at telomeres in the macronuclei of ciliated protozoans, with special attention given to studies in Oxytricha (a hypotrichous ciliate) and Tetrahymena (a holotrichous ciliate). The structure of telomeres has been largely elucidated by studies on macronuclear DNA from ciliated protozoans. Studies of Tetrahymena recombinant DNA (rDNA) termini provided the first telomeric DNA sequence. Short tandem repeats, very similar to those described in Tetrahymena, have since been detected at telomeres in a wide variety of lower eukaryotes. The Tetrahymena rDNA termini also provided the first indication that telomeres exist in a non-nucleosomal DNA–protein complex. Subsequent studies from Oxytricha macronuclei have generated further insights into telomere structure. The terminal ∼135 bp of Oxytricha macronuclear DNA molecules are packaged in a non-nucleosomal protein complex. Internal to the non-nucleosomal telomeric complex are several precisely positioned nucleosomes, a phenomenon that has been observed on the extrachromosomal rDNA molecules from Tetrahymena and Dictyostelium.
AB - This chapter discusses the DNA–protein complexes found at telomeres in the macronuclei of ciliated protozoans, with special attention given to studies in Oxytricha (a hypotrichous ciliate) and Tetrahymena (a holotrichous ciliate). The structure of telomeres has been largely elucidated by studies on macronuclear DNA from ciliated protozoans. Studies of Tetrahymena recombinant DNA (rDNA) termini provided the first telomeric DNA sequence. Short tandem repeats, very similar to those described in Tetrahymena, have since been detected at telomeres in a wide variety of lower eukaryotes. The Tetrahymena rDNA termini also provided the first indication that telomeres exist in a non-nucleosomal DNA–protein complex. Subsequent studies from Oxytricha macronuclei have generated further insights into telomere structure. The terminal ∼135 bp of Oxytricha macronuclear DNA molecules are packaged in a non-nucleosomal protein complex. Internal to the non-nucleosomal telomeric complex are several precisely positioned nucleosomes, a phenomenon that has been observed on the extrachromosomal rDNA molecules from Tetrahymena and Dictyostelium.
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U2 - 10.1016/S1569-2558(08)60438-5
DO - 10.1016/S1569-2558(08)60438-5
M3 - Article
AN - SCOPUS:0344716729
SN - 1569-2558
VL - 2
SP - 291
EP - 307
JO - Advances in Molecular and Cell Biology
JF - Advances in Molecular and Cell Biology
IS - C
ER -