DNA binding sites for the transcriptional activator/repressor YY1

Robin P. Hyde-deruyscher, Ezra Jennings, Thomas Shenk

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

YY1 is a ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the Initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported In a wide variety of promoters and they exhibit substantial diversity in their sequence. To better understand how YY1 interacts with DNA and to be able to predict the presence of YY1 sites In a more comprehensive fashion, we have selected YY1 binding sites from a random pool of oligonucleotides. The sites display considerable heterogeneity, but contain a conserved 5′-CAT-3′ core flanked by variable regions, generating the consensus 5′-{C/g/a)(G/l)(C/t/a)CATN(T/a)T/g/c)-3′, where the upper case letters represent the preferred base. This high degree of flexibility in DNA recognition can be predicted by modeling the Interaction of the four YY1 zinc fingers with DNA and a detailed model for this interaction is presented and discussed.

Original languageEnglish (US)
Pages (from-to)4457-4465
Number of pages9
JournalNucleic acids research
Volume23
Issue number21
DOIs
StatePublished - 1995

All Science Journal Classification (ASJC) codes

  • Genetics

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