DNA assembly for nanopore data storage readout

Randolph Lopez, Yuan Jyue Chen, Siena Dumas Ang, Sergey Yekhanin, Konstantin Makarychev, Miklos Z. Racz, Georg Seelig, Karin Strauss, Luis Ceze

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Synthetic DNA is becoming an attractive substrate for digital data storage due to its density, durability, and relevance in biological research. A major challenge in making DNA data storage a reality is that reading DNA back into data using sequencing by synthesis remains a laborious, slow and expensive process. Here, we demonstrate successful decoding of 1.67 megabytes of information stored in short fragments of synthetic DNA using a portable nanopore sequencing platform. We design and validate an assembly strategy for DNA storage that drastically increases the throughput of nanopore sequencing. Importantly, this assembly strategy is generalizable to any application that requires nanopore sequencing of small DNA amplicons.

Original languageEnglish (US)
Article number2933
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Lopez, R., Chen, Y. J., Dumas Ang, S., Yekhanin, S., Makarychev, K., Racz, M. Z., Seelig, G., Strauss, K., & Ceze, L. (2019). DNA assembly for nanopore data storage readout. Nature communications, 10(1), [2933]. https://doi.org/10.1038/s41467-019-10978-4