Diversity of culturable denitrifying bacteria - Limits of rDNA RFLP analysis and probes for the functional gene, nitrite reductase

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Abstract

Sequence divergence in the ribosomal genes of known strains and isolates of aquatic denitrifying bacteria was investigated using restriction fragment length polymorphism (RFLP) analysis. The same cultures were characterized for their homology with antibody and gene probes for nitrite reductase (NiR), a key enzyme in the denitrification pathway, and for amplification with a set of polymerase chain reaction primers designed to amplify a portion of the NiR gene. The NiR probes were developed from Pseudomonas stutzeri (ATCC 14405) and several P. stutzeri strains were included in the analyses. The RFLP analysis clustered most of the P. stutzeri strains together, but detected considerable diversity within this group. Isolates from three aquatic environments exhibited within -and among - habitat diversity by RFLP. Hybridization with the NiR probes and amplification with the NiR primers were not correlated with the clustering of strains by rDNA RFLP analysis. The relationships among strains deduced from ribosomal DNA RFLP reflect heterogeneity within the P. stutzeri group and among other pseudomonads, and the patterns differ from those inferred from specificity of the NiR probes.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalArchives of Microbiology
Volume163
Issue number3
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Keywords

  • Denitrifying bacteria
  • Diversity
  • Nitrite reductase
  • Restriction fragment length polymorphism

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