@article{4a0c3a3b23ff462fbcf1f7e054ec1935,
title = "Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome",
abstract = "Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon{\textquoteright}s MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome.",
author = "Conway, {Jonathan M.} and Walton, {William G.} and Isai Salas-Gonz{\'a}lez and Law, {Theresa F.} and Lindberg, {Chloe A.} and Crook, {Laura E.} and Kosina, {Suzanne M.} and Fitzpatrick, {Connor R.} and Lietzan, {Adam D.} and Northen, {Trent R.} and Jones, {Corbin D.} and Finkel, {Omri M.} and Redinbo, {Matthew R.} and Dangl, {Jeffery L.}",
note = "Funding Information: J.L.D. is a co-founder of, and shareholder in, AgBiome LLC, a corporation with the goal of using plant-associated microorganisms to improve plant productivity. M.R.R. is co-founder of Symberix, Inc., and receives research funding from Merck and Lilly. The remaining authors declare no competing interests. Funding Information: This work was supported by NSF grant IOS-1917270 and by Office of Science (BER), US Department of Energy Grant DE-SC0014395 to J.L.D. J.L.D. is an Investigator of the Howard Hughes Medical Institute, supported by the HHMI. T.R.N. and S.M.K. gratefully acknowledge funding from the m-CAFEs Microbial Community Analysis & Functional Evaluation in Soils Science Focus Area which is supported by the US Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 to Lawrence Berkeley National Laboratory. We thank J. Leveau (University of California Davis) and P. Schulze-Lefert (Max-Planck-Gesellschaft) for providing bacterial strains used in this work. This article is subject to HHMI{\textquoteright}s Open Access to Publications policy. HHMI lab heads have previously granted a non-exclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication. Funding Information: This work was supported by NSF grant IOS-1917270 and by Office of Science (BER), US Department of Energy Grant DE-SC0014395 to J.L.D. J.L.D. is an Investigator of the Howard Hughes Medical Institute, supported by the HHMI. T.R.N. and S.M.K. gratefully acknowledge funding from the m-CAFEs Microbial Community Analysis & Functional Evaluation in Soils Science Focus Area which is supported by the US Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 to Lawrence Berkeley National Laboratory. We thank J. Leveau (University of California Davis) and P. Schulze-Lefert (Max-Planck-Gesellschaft) for providing bacterial strains used in this work. This article is subject to HHMI{\textquoteright}s Open Access to Publications policy. HHMI lab heads have previously granted a non-exclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = nov,
doi = "10.1038/s41564-022-01244-3",
language = "English (US)",
volume = "7",
pages = "1817--1833",
journal = "Nature Microbiology",
issn = "2058-5276",
publisher = "Nature Publishing Group",
number = "11",
}