Distinctions between the two state and sequential models for cooperative ligand binding

The two state and sequential models for positive cooperativity in ligand binding can produce significantly different theoretical binding curves when presented in a Scatchard plot. The conditions that produce the greatest differences have been examined. The theoretical differences have been used to select the two state model as the best model for describing the binding of acetylcholine to acetylcholine receptors that have been solubilized by Triton X 100 and sodium cholate.