@article{4cfa41dd513c4c68a4ebc52a47e78bdc,
title = "Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in helicobacter pylori",
abstract = "Helical cell shape is necessary for efficient stomach colonization by Helicobacter pylori, but the molecular mechanisms for generating helical shape remain unclear. The helical centerline pitch and radius of wild-type H. pylori cells dictate surface curvatures of considerably higher positive and negative Gaussian curvatures than those present in straight-or curved-rod H. pylori. Quantitative 3D microscopy analysis of short pulses with either N-acetylmuramic acid or D-alanine metabolic probes showed that cell wall growth is enhanced at both sidewall curvature extremes. Immunofluorescence revealed MreB is most abundant at negative Gaussian curvature, while the bactofilin CcmA is most abundant at positive Gaussian curvature. Strains expressing CcmA variants with altered polymerization properties lose helical shape and associated positive Gaussian curvatures. We thus propose a model where CcmA and MreB promote PG synthesis at positive and negative Gaussian curvatures, respectively, and that this patterning is one mechanism necessary for maintaining helical shape.",
author = "Taylor, {Jennifer A.} and Bratton, {Benjamin P.} and Sichel, {Sophie R.} and Blair, {Kris M.} and Jacobs, {Holly M.} and Demeester, {Kristen E.} and Erkin Kuru and Joe Gray and Jacob Biboy and Vannieuwenhze, {Michael S.} and Waldemar Vollmer and Grimes, {Catherine L.} and Shaevitz, {Joshua W.} and Salama, {Nina R.}",
note = "Funding Information: This research was supported in part by US National Institutes of Health R01 AI136946 (NRS), U01 CA221230 (CLG and NRS), T32 CA009657 (KMB), T32 GM95421 (SRS), T32 GM008550 (KED), GM113172 (MSV) the FHCRC Cellular Imaging and Genomics and Bioinformatics Shared Resources of the NCI Center Support Grant P30 CA015704, the Stanford Imaging Award Number 1S10OD01227601 from the National Center for Research Resources (NCRR), the Life Sciences Research Foundation (EK), and the Wellcome Trust grant 101824/Z/13/Z (WV).This work was supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. DGE-0718124 (JAT) and DGE-1256082 (JAT and KMB), the Department of Defense (DoD) through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program (JAT), and the GO-MAP Graduate Opportunity Program Research Assistantship Award (GOP Award) (SRS). This work was supported by National Science Foundation PHY-1734030 (BPB and JWS), the Glenn Centers for Aging Research (BPB) and National Institutes of Health NIH R21 AI121828 (BPB and JWS). The opinions, findings, and conclusions or recommendations expressed in this material contents are solely the responsibility of the authors and do not necessarily represent the official views of the NCRR, the National Institutes of Health, the Department of Defense, or the National Science Foundation. The authors have no conflicts of interest to report. We would like to thank Laura Sycuro, Desir?e Yang, and Irina Mavrodi for strain construction; Dr. Cintia Santiago for assistance with NAM purification and compound shipment; Patrina Pellett (GE Healthcare) for assistance with OMX imaging; the David Baker Lab (University of Washington) for OMX access and use; and Sloan Siegrist (University of Massachusetts Amhurst) for the D-alanine-D-alanine-alkyne and D-alanine-alkyne-D-alanine reagents. We kindly thank Dr. Hong Wu and Dr. Kouichi Sano (Osaka Medical College) for the anti-MreB and corresponding preimmune sera used in this study; Anson Chan (University of British Columbia) for consultation on CcmA mutant design; and Zachary Jones for assistance in synthesizing the MurNAc sugars. Publisher Copyright: {\textcopyright} 2020, eLife Sciences Publications Ltd. All rights reserved.",
year = "2020",
month = jan,
doi = "10.7554/eLife.52482",
language = "English (US)",
volume = "9",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}