Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics

François Courvoisier; Véronique Boutou; Laurent Guyon; Matthias Roth; Herschel Rabitz; Jean Pierre Wolf

doi:10.1016/j.jphotochem.2006.03.039

Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics

François Courvoisier, Véronique Boutou, Laurent Guyon, Matthias Roth, Herschel Rabitz, Jean Pierre Wolf

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.

Original language	English (US)
Pages (from-to)	300-306
Number of pages	7
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	180
Issue number	3
DOIs	https://doi.org/10.1016/j.jphotochem.2006.03.039
State	Published - Jun 25 2006

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)
Physics and Astronomy(all)

Keywords

Aerosol particles discrimination
Bio-molecules ultrafast spectroscopy
Biological intrinsic fluorophore
Femtosecond fluorescence depletion
Real-time particle detection

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10.1016/j.jphotochem.2006.03.039

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title = "Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics",

abstract = "We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus f{\ae}calis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.",

keywords = "Aerosol particles discrimination, Bio-molecules ultrafast spectroscopy, Biological intrinsic fluorophore, Femtosecond fluorescence depletion, Real-time particle detection",

author = "Fran{\c c}ois Courvoisier and V{\'e}ronique Boutou and Laurent Guyon and Matthias Roth and Herschel Rabitz and Wolf, {Jean Pierre}",

note = "Funding Information: J.-P.W. thanks P. Callis, S.C. Hill and P. Hargis for fruitful discussions. F.C., V.B., and J.-P.W. acknowledge DGA's grant 036000036004707501 and la R{\'e}gion Rh{\^o}ne-Alpes for its support through Emergence . H.R. acknowledges the support of the Army Research Office and the DoD Multidisciplinary University Research Initiative Program. J-P.W. acknowledges NATO's grant SSTCLG977928. ",

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doi = "10.1016/j.jphotochem.2006.03.039",

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AU - Courvoisier, François

AU - Boutou, Véronique

AU - Guyon, Laurent

AU - Roth, Matthias

AU - Rabitz, Herschel

AU - Wolf, Jean Pierre

N1 - Funding Information: J.-P.W. thanks P. Callis, S.C. Hill and P. Hargis for fruitful discussions. F.C., V.B., and J.-P.W. acknowledge DGA's grant 036000036004707501 and la Région Rhône-Alpes for its support through Emergence . H.R. acknowledges the support of the Army Research Office and the DoD Multidisciplinary University Research Initiative Program. J-P.W. acknowledges NATO's grant SSTCLG977928.

PY - 2006/6/25

Y1 - 2006/6/25

N2 - We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.

AB - We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.

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KW - Bio-molecules ultrafast spectroscopy

KW - Biological intrinsic fluorophore

KW - Femtosecond fluorescence depletion

KW - Real-time particle detection

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Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics

Abstract

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