TY - JOUR
T1 - Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics
AU - Courvoisier, François
AU - Boutou, Véronique
AU - Guyon, Laurent
AU - Roth, Matthias
AU - Rabitz, Herschel
AU - Wolf, Jean Pierre
N1 - Funding Information:
J.-P.W. thanks P. Callis, S.C. Hill and P. Hargis for fruitful discussions. F.C., V.B., and J.-P.W. acknowledge DGA's grant 036000036004707501 and la Région Rhône-Alpes for its support through Emergence . H.R. acknowledges the support of the Army Research Office and the DoD Multidisciplinary University Research Initiative Program. J-P.W. acknowledges NATO's grant SSTCLG977928.
PY - 2006/6/25
Y1 - 2006/6/25
N2 - We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.
AB - We investigated femtosecond pump-repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.
KW - Aerosol particles discrimination
KW - Bio-molecules ultrafast spectroscopy
KW - Biological intrinsic fluorophore
KW - Femtosecond fluorescence depletion
KW - Real-time particle detection
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U2 - 10.1016/j.jphotochem.2006.03.039
DO - 10.1016/j.jphotochem.2006.03.039
M3 - Article
AN - SCOPUS:33744547100
VL - 180
SP - 300
EP - 306
JO - Journal of Photochemistry and Photobiology A: Chemistry
JF - Journal of Photochemistry and Photobiology A: Chemistry
SN - 1010-6030
IS - 3
ER -