Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning

Kevin Welsher, Simon A. McManus, Chih Hao Hsia, Shuhui Yin, Haw Yang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The seemingly inevitable protein corona appears to be an insurmountable obstacle to wider application of functional nanomaterials in biotechnology. The accumulation of serum proteins can block targeting functionalities and alter the in vivo fate of these nanomaterials. Here we demonstrate a method to generate non-stick, robustly passivated functional nanoparticles (NPs) using a tailored silica coating. We apply agarose gel electrophoresis to sensitively evaluate protein binding to NPs with different surface chemistry. Using gel banding and retardation as a read-out for protein adsorption, we optimize the surface chemistry to yield a mixed charge surface which displays remarkable binding resistance to a wide range of serum proteins and nucleic acids. The hard silica shell also protects the functional NP core in harsh environments (down to pH 1) while still showing the ability to be targeted for cellular uptake with little or no non-specific binding.

Original languageEnglish (US)
Pages (from-to)580-583
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number2
DOIs
StatePublished - Jan 21 2015

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning'. Together they form a unique fingerprint.

Cite this