Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas

Joshua Jack; Jonathan Lo; Pin Ching Maness; Zhiyong Jason Ren

doi:10.1016/j.biombioe.2019.03.011

Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas

Joshua Jack, Jonathan Lo, Pin Ching Maness, Zhiyong Jason Ren

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H₂/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H₂/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H₂/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H₂/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

Original language	English (US)
Pages (from-to)	95-101
Number of pages	7
Journal	Biomass and Bioenergy
Volume	124
DOIs	https://doi.org/10.1016/j.biombioe.2019.03.011
State	Published - May 2019

All Science Journal Classification (ASJC) codes

Forestry
Renewable Energy, Sustainability and the Environment
Agronomy and Crop Science
Waste Management and Disposal

Keywords

Clostridium ljungdahlii
Fermentation
H/CO ratio
Liquid fuel
Syngas

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10.1016/j.biombioe.2019.03.011

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title = "Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas",

abstract = "Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.",

keywords = "Clostridium ljungdahlii, Fermentation, H/CO ratio, Liquid fuel, Syngas",

author = "Joshua Jack and Jonathan Lo and Maness, {Pin Ching} and Ren, {Zhiyong Jason}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = may,

doi = "10.1016/j.biombioe.2019.03.011",

language = "English (US)",

volume = "124",

pages = "95--101",

journal = "Biomass and Bioenergy",

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T1 - Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas

AU - Jack, Joshua

AU - Lo, Jonathan

AU - Maness, Pin Ching

AU - Ren, Zhiyong Jason

PY - 2019/5

Y1 - 2019/5

N2 - Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

AB - Clostridium ljungdahlii was grown on syngas of varying composition to investigate how syngas blends used in fermentation can be tailored toward desired product formation. Experiments were conducted with liquid batch cultures exposed to the same volume of gas with varying ratios of hydrogen to carbon monoxide (H2/CO). Formation of acetate was favored by higher concentrations of hydrogen in the headspace. The maximum acetate concentration of 35.21 ± 0.84 mM was obtained using an H2/CO ratio of 2.0. Generation of more reduced metabolites was favored by greater carbon monoxide concentrations in the headspace. Maximum ethanol (7.53 ± 0.58 mM) and 2,3-Butanediol (5.20 ± 0.79 mM) concentrations were achieved under an H2/CO ratio of 0.5. Linear relationships were obtained in that describe the effect of the syngas H2/CO ratios on acetate, ethanol, and 2,3-BD formation, respectively.

KW - Clostridium ljungdahlii

KW - Fermentation

KW - H/CO ratio

KW - Liquid fuel

KW - Syngas

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VL - 124

SP - 95

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JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

ER -

Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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