Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy

Heng Ni, Xiao Fan, Feng Zhou, Galio Guo, Jae Young Lee, Nadrian C. Seeman, Do Nyun Kim, Nan Yao, Paul M. Chaikin, Yimo Han

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Two-dimensional (2D) DNA origami that is capable of self-assembling into complex 2D and 3D geometries pave the way for a bottom-up synthesis for various applications in nano/biotechnology. Here, we directly visualized the aqueous structure of 2D DNA origami cross-tiles and their assemblies using cryogenic electron microscopy. We uncovered flexible arms in cross-tile monomers and designated inter-tile folding. In addition, we observed the formation of clusters and stacks of DNA cross-tiles in solution, which could potentially affect the interaction and assembly of DNA origami. Finally, we quantitatively evaluated the flexibility of DNA origami in solution using finite element analysis. Our discovery has laid the foundation for investigating the dynamic structures of 2D DNA origami assemblies in solution, providing insights regarding the self-assembly and self-replication mechanisms of 2D DNA origami.

Original languageEnglish (US)
Article number104373
Issue number6
StatePublished - Jun 17 2022

All Science Journal Classification (ASJC) codes

  • General


  • Biomaterials
  • Materials characterization
  • Materials chemistry
  • Materials science


Dive into the research topics of 'Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy'. Together they form a unique fingerprint.

Cite this