@article{7326545fc69646d59945bdc6cc23666d,
title = "Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy",
abstract = "Two-dimensional (2D) DNA origami that is capable of self-assembling into complex 2D and 3D geometries pave the way for a bottom-up synthesis for various applications in nano/biotechnology. Here, we directly visualized the aqueous structure of 2D DNA origami cross-tiles and their assemblies using cryogenic electron microscopy. We uncovered flexible arms in cross-tile monomers and designated inter-tile folding. In addition, we observed the formation of clusters and stacks of DNA cross-tiles in solution, which could potentially affect the interaction and assembly of DNA origami. Finally, we quantitatively evaluated the flexibility of DNA origami in solution using finite element analysis. Our discovery has laid the foundation for investigating the dynamic structures of 2D DNA origami assemblies in solution, providing insights regarding the self-assembly and self-replication mechanisms of 2D DNA origami.",
keywords = "Biomaterials, Materials characterization, Materials chemistry, Materials science",
author = "Heng Ni and Xiao Fan and Feng Zhou and Galio Guo and Lee, {Jae Young} and Seeman, {Nadrian C.} and Kim, {Do Nyun} and Nan Yao and Chaikin, {Paul M.} and Yimo Han",
note = "Funding Information: G.G. and Y.H. are supported by a Research grant from Welch Foundation (Grant #: C-2065-20210327). F.Z. acknowledges DOE DE-SC0007991 for DNA and origami synthesis. H.N. acknowledges the Center for Bio-Inspired Energy Sciences, an Energy Frontier Research Center funded by the DOE, Office of Sciences, Basic Energy Sciences, under award no. DE-SC0000989 for the simulation of the origami structure. J.Y. L. and D.-N. K. are supported by the National Convergence Research of Scientific Challenges (NRF-2020M3F7A1094299) and the Basic Research Program (NRF-2019R1A2C4069541) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. The authors acknowledge the use of Princeton's Imaging and Analysis Center, which is partially supported by the Princeton Center for Complex Materials, a National Science Foundation (NSF)-MRSEC program (DMR-2011750). This work also made use of the Electron Microscopy Center at Rice University. We thank Mark Bathe for help on the CanDo simulation (Castro et al. 2011; Kim et al. 2012). We thank Ruojie Sha and Chuqiao Shi for their helpful discussion. Conceptualization, Y.H. and F.Z.; Methodology, H.N. X.F. F.Z. G.G. J.Y.L. and D.-N.K.; Investigation, Y.H. H.N. X.F. and F.Z.; Writing – Original Draft, Y.H. H.N. F.Z. and G.G.; Review & Editing, Y.H. H.N. X.F. and F.Z.; Funding Acquisition, Y.H. N.Y. and P.C.; Resources, N.Y.; Supervision, Y.H. P.C. and N.Y. The authors present no competing interests. Funding Information: G.G. and Y.H. are supported by a Research grant from Welch Foundation (Grant #: C-2065-20210327 ). F.Z. acknowledges DOE DE-SC0007991 for DNA and origami synthesis. H.N. acknowledges the Center for Bio-Inspired Energy Sciences , an Energy Frontier Research Center funded by the DOE , Office of Sciences, Basic Energy Sciences , under award no. DE-SC0000989 for the simulation of the origami structure. J.Y. L. and D.-N. K. are supported by the National Convergence Research of Scientific Challenges ( NRF - 2020M3F7A1094299 ) and the Basic Research Program ( NRF - 2019R1A2C4069541 ) through the National Research Foundation of Korea ( NRF ) funded by the Ministry of Science and ICT . The authors acknowledge the use of Princeton{\textquoteright}s Imaging and Analysis Center , which is partially supported by the Princeton Center for Complex Materials , a National Science Foundation ( NSF )- MRSEC program ( DMR-2011750 ). This work also made use of the Electron Microscopy Center at Rice University . We thank Mark Bathe for help on the CanDo simulation ( Castro et al., 2011 ; Kim et al., 2012 ). We thank Ruojie Sha and Chuqiao Shi for their helpful discussion. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.isci.2022.104373",
language = "English (US)",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "6",
}