@article{7d0f1c0626a94c9c91419d660ab2d478,
title = "Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology",
abstract = "When 3D electron microscopy and calcium imaging are used to investigate the structure and function of neural circuits, the resulting datasets pose new challenges of visualization and interpretation. Here, we present a new kind of digital resource that encompasses almost 400 ganglion cells from a single patch of mouse retina. An online “museum” provides a 3D interactive view of each cell's anatomy, as well as graphs of its visual responses. The resource reveals two aspects of the retina's inner plexiform layer: an arbor segregation principle governing structure along the light axis and a density conservation principle governing structure in the tangential plane. Structure is related to visual function; ganglion cells with arbors near the layer of ganglion cell somas are more sustained in their visual responses on average. Our methods are potentially applicable to dense maps of neuronal anatomy and physiology in other parts of the nervous system. A digital “museum” of retinal ganglion cells combines dense maps of anatomy and physiology at cellular resolution. The inner plexiform layer of the retina divides into four sublaminae defined by a purely anatomical principle of arbor segregation. We test the hypothesis that the aggregate neurite density of a ganglion cell type should be approximately uniform (“density conservation”). Ganglion cells arborizing in the inner marginal sublamina of the inner plexiform layer exhibit significantly more sustained visual responses on average.",
keywords = "3D reconstruction, calcium imaging, cell type, crowdsourcing, electron microscopy, ganglion cell, inner plexiform layer, mouse, online atlas, retina",
author = "{the EyeWirers} and Bae, {J. Alexander} and Shang Mu and Kim, {Jinseop S.} and Turner, {Nicholas L.} and Ignacio Tartavull and Nico Kemnitz and Jordan, {Chris S.} and Norton, {Alex D.} and Silversmith, {William M.} and Rachel Prentki and Marissa Sorek and Celia David and Jones, {Devon L.} and Doug Bland and Sterling, {Amy L.R.} and Jungman Park and Briggman, {Kevin L.} and Seung, {Hyunjune Sebastian}",
note = "Funding Information: We are grateful to W. Denk for providing the e2198 dataset. We thank C.-G. Hwang for originating the idea of collaboration between KT Corporation and Eyewire on “citizen neuroscience” and to H. Park for helping to make the connection. S. Caddick and D. Feshbach provided guidance to WiredDifferently. S. Str{\"o}h helped identify correspondences with cell types in the literature. M.J. Greene helped start the contact analysis and skeletonization. M. Balkam and K. Radul assisted with Eyewire in the early stages of this investigation. M. Kim, K. Lee, and D. Ih helped manage the Korean Eyewire community. B. Paiva contributed Eyewire logo animations. C. Xiang and N. Benson created badges and art for promoting competitions. N. Friedman created educational material for the Eyewire blog and wiki. C. O{\textquoteright}Toole and D. Sparer promoted Eyewire on social media and blogs. M. Akasako contributed material on cell types to the wiki. We benefited from interactions with P. Berens, M. Berry, D. Berson, B. Borghuis, T. Euler, J. Homann, M. Meister, T. Schmidt, G. Schwartz, and J. Sanes. Research was supported by the Gatsby Charitable Foundation , NINDS/NIH ( U01NS090562 and 5R01NS076467 ), DARPA ( HR0011-14-2-0004 ), ARO ( W911NF-12-1-0594 ), IARPA ( D16PC00005 ), KT Corporation , and the Amazon Web Services Research Grants Program. J.S.K. acknowledges support from Korea Brain Research Institute Basic Research Program ( 2231-415 ) and Brain Research Program through the National Research Foundation ( 2017M3C7A1048086 ) both funded by the Korea Ministry of Science and ICT . Funding Information: We are grateful to W. Denk for providing the e2198 dataset. We thank C.-G. Hwang for originating the idea of collaboration between KT Corporation and Eyewire on “citizen neuroscience” and to H. Park for helping to make the connection. S. Caddick and D. Feshbach provided guidance to WiredDifferently. S. Str{\"o}h helped identify correspondences with cell types in the literature. M.J. Greene helped start the contact analysis and skeletonization. M. Balkam and K. Radul assisted with Eyewire in the early stages of this investigation. M. Kim, K. Lee, and D. Ih helped manage the Korean Eyewire community. B. Paiva contributed Eyewire logo animations. C. Xiang and N. Benson created badges and art for promoting competitions. N. Friedman created educational material for the Eyewire blog and wiki. C. O'Toole and D. Sparer promoted Eyewire on social media and blogs. M. Akasako contributed material on cell types to the wiki. We benefited from interactions with P. Berens, M. Berry, D. Berson, B. Borghuis, T. Euler, J. Homann, M. Meister, T. Schmidt, G. Schwartz, and J. Sanes. Research was supported by the Gatsby Charitable Foundation, NINDS/NIH (U01NS090562 and 5R01NS076467), DARPA (HR0011-14-2-0004), ARO (W911NF-12-1-0594), IARPA (D16PC00005), KT Corporation, and the Amazon Web Services Research Grants Program. J.S.K. acknowledges support from Korea Brain Research Institute Basic Research Program (2231-415) and Brain Research Program through the National Research Foundation (2017M3C7A1048086) both funded by the Korea Ministry of Science and ICT. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = may,
day = "17",
doi = "10.1016/j.cell.2018.04.040",
language = "English (US)",
volume = "173",
pages = "1293--1306.e19",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}