Differential Allele-Specific Expression Uncovers Breast Cancer Genes Dysregulated by Cis Noncoding Mutations

Pawel F. Przytycki, Mona Singh

Research output: Contribution to journalArticle

Abstract

Identifying cancer-relevant mutations in noncoding regions is challenging due to the large numbers of such mutations, their low levels of recurrence, and difficulties in interpreting their functional impact. To uncover genes that are dysregulated due to somatic mutations in cis, we build upon the concept of differential allele-specific expression (ASE) and introduce methods to identify genes within an individual's cancer whose ASE differs from what is found in matched normal tissue. When applied to breast cancer tumor samples, our methods detect the known allele-specific effects of copy number variation and nonsense-mediated decay. Further, genes that are found to recurrently exhibit differential ASE across samples are cancer relevant. Genes with cis mutations are enriched for differential ASE, and we find 147 potentially functional noncoding mutations cis to genes that exhibit significant differential ASE. We conclude that differential ASE is a promising means for discovering gene dysregulation due to cis noncoding mutations. Przytycki et al. introduce methods to uncover differential allele-specific expression in heterogeneous tumor samples and apply them to identify potentially functional cis noncoding mutations in breast cancer.

Original languageEnglish (US)
Pages (from-to)193-203.e4
JournalCell Systems
Volume10
Issue number2
DOIs
StatePublished - Feb 26 2020

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Keywords

  • allele-specific expression
  • cancer
  • noncoding mutations
  • whole-exome sequencing
  • whole-genome sequencing

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