TY - JOUR
T1 - Developmental regulation of vesicle transport in Drosophila embryos
T2 - Forces and kinetics
AU - Welte, Michael A.
AU - Gross, Steven P.
AU - Postner, Marya
AU - Block, Steven M.
AU - Wieschaus, Eric F.
N1 - Funding Information:
M. A. W. thanks S. Kramer and Y. Ahmed for technical advice. S. P. G. is a recipient of NIH postdoctoral traineeship 5F32GM18329 from the NIGMS and acknowledges helpful discussions with M. Schnitzer and K. Visscher. M. P. was supported by a predoctoral traineeship from the NIGMS. S. M. B. and S. P. G. were supported by grant 5R01GM5143 from the NIGMS. E. F. W. and M. A. W. gratefully acknowledge support from the Howard Hughes Medical Institute and grant 5R37HD15587 from the NICHD. The authors thank J. Fischer-Vize and C. Nüsslein-Volhard for providing fly strains, and J. Fischer-Vize for generously sharing unpublished data on marbles .
PY - 1998/2/20
Y1 - 1998/2/20
N2 - In Drosophila embryos, microtubules oriented along apical-basal directions support saltatory vesicle movement. Vesicle traffic includes lipid droplets whose distribution shifts twice during early embryogenesis. Using microscopy, optical tweezers, and a novel squashed-mount embryo preparation, we tracked single droplets and measured the forces these generated. Droplet stalling forces change developmentally, in a roughly quantized fashion, consistent with variation in the number of active motors. We characterized a mutation, klarsicht, that affects droplet transport. Klar+ facilitates changes in force, possibly by coordinating the activity of multiple motors. Alterations in transport affected motion in both apical and basal directions, indicating fight coupling between motors of opposite polarity. Mutations in klar also affect nuclear migration during eye development, suggesting multiple roles for klar-based transport.
AB - In Drosophila embryos, microtubules oriented along apical-basal directions support saltatory vesicle movement. Vesicle traffic includes lipid droplets whose distribution shifts twice during early embryogenesis. Using microscopy, optical tweezers, and a novel squashed-mount embryo preparation, we tracked single droplets and measured the forces these generated. Droplet stalling forces change developmentally, in a roughly quantized fashion, consistent with variation in the number of active motors. We characterized a mutation, klarsicht, that affects droplet transport. Klar+ facilitates changes in force, possibly by coordinating the activity of multiple motors. Alterations in transport affected motion in both apical and basal directions, indicating fight coupling between motors of opposite polarity. Mutations in klar also affect nuclear migration during eye development, suggesting multiple roles for klar-based transport.
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U2 - 10.1016/S0092-8674(00)80947-2
DO - 10.1016/S0092-8674(00)80947-2
M3 - Article
C2 - 9491895
AN - SCOPUS:0032548920
SN - 0092-8674
VL - 92
SP - 547
EP - 557
JO - Cell
JF - Cell
IS - 4
ER -