Developmental changes of FABP concentration, expression, and intracellular distribution in locust flight muscle

Norbert H. Haunerland, Xinmei Chen, Peter Andolfatto, Joan M. Chisholm, Zhixiang Wang

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

M-FABP from flight muscle of the locust, Schistocerca gregaria, is similar to mammalian H-FABP in its physical characteristics and amino acid sequence. We have studied developmental changes using ELISA, Northern Blotting, and EM/immuno-gold techniques. M-FABP is found in cytoplasm and nuclei, but not in mitochondria. It is the most abundant soluble muscle protein in fully developed adult locusts, comprising 18% of the total cytosolic protein. However, no FABP is detectable at the beginning of the adult stage. Its concentration rises dramatically during the next 10 days, after which it reaches its maximal value. Expression apparently is turned on after adult ecdysis and continues for 10 days; thereafter, FABP mRNA diminishes and reaches a constant, but low level, probably needed to maintain the current FABP level. From a series of experiments employing metamorphosis-controlling hormones and antihormones it is evident that the induction of FABP expression is directly linked to metamorphosis.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume123
Issue number1-2
DOIs
StatePublished - Jun 1 1993

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Keywords

  • fatty acid-binding protein
  • immuno-gold labeling
  • insect metamorphosis
  • nuclear protein

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