Deterministic separation of cancer cells from blood at 10 mL/min

Kevin Loutherback, Joseph D'Silva, Liyu Liu, Amy Wu, Robert H. Austin, James C. Sturm

Research output: Contribution to journalArticle

149 Scopus citations

Abstract

Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability.

Original languageEnglish (US)
Article number042107
JournalAIP Advances
Volume2
Issue number4
DOIs
StatePublished - 2012

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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