Deterministic hydrodynamics: Taking blood apart

John A. Davis, David W. Inglis, Keith J. Morton, David A. Lawrence, Lotien R. Huang, Stephen Y. Chou, James C. Sturm, Robert H. Austin

Research output: Contribution to journalArticlepeer-review

432 Scopus citations

Abstract

We show the fractionation of whole blood components and isolation of blood plasma with no dilution by using a continuous-flow deterministic array that separates blood components by their hydrodynamic size, independent of their mass. We use the technology we developed of deterministic arrays which separate white blood cells, red blood cells, and platelets from blood plasma at flow velocities of 1,000 μm/sec and volume rates up to 1 μl/min. We verified by flow cytometry that an array using focused injection removed 100% of the lymphocytes and monocytes from the main red blood cell and platelet stream. Using a second design, we demonstrated the separation of blood plasma from the blood cells (white, red, and platelets) with virtually no dilution of the plasma and no cellular contamination of the plasma.

Original languageEnglish (US)
Pages (from-to)14779-14784
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number40
DOIs
StatePublished - Oct 3 2006

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Cells
  • Microfabrication
  • Plasma
  • Separation

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