Determining the role of matrix compliance in the differentiation of mammary stem cells

Kangae Lee, Celeste M. Nelson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multipotent stem cells maintain the structure and function of the mammary gland throughout its development and respond to the physiological demands associated with pregnancy and lactation. The ability of mammary stem cells to maintain themselves as well as to give rise to differentiated progeny is not only affected by soluble factors but has increasingly become linked to mechanical cues including the elastic modulus of the extracellular matrix (ECM). Here we describe a protocol for determining how the mechanical properties of the ECM regulate the fate of mammary stem or progenitor cells. This protocol includes detailed methods for the fabrication of substrata with varying stiffness, culture of mammary progenitor cells on synthetic substrata, pharmacological modulation of actomyosin contractility, and analysis of gene expression to define the resulting fate of human mammary stem cells.

Original languageEnglish (US)
Title of host publicationBiomimetics and Stem Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages79-94
Number of pages16
ISBN (Print)9781493913312
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1202
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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