Determining drug release rates of hydrophobic compounds from nanocarriers

Suzanne M. D'Addio, Abdallah A. Bukari, Mohammed Dawoud, Heike Bunjes, Carlos Rinaldi, Robert K. Prud'Homme

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Obtaining meaningful drug release profiles for drug formulations is essential prior to in vivo testing and for ensuring consistent quality. The release kinetics of hydrophobic drugs from nanocarriers (NCs) are not well understood because the standard protocols for maintaining sink conditions and sampling are not valid owing to mass transfer and solubility limitations. In this work, a new in vitro assay protocol based on 'lipid sinks' and magnetic separation produces release conditions that mimic the concentrations of lipid membranes and lipoproteins in vivo, facilitates separation, and thus allows determination of intrinsic release rates of drugs from NCs. The assay protocol is validated by (i) determining the magnetic separation efficiency, (ii) demonstrating that sink condition requirements are met, and (iii) accounting for drug by completing a mass balance. NCs of itraconazole and cyclosporine A (CsA) were prepared and the drug release profiles were determined. This release protocol has been used to compare the drug release from a polymer stabilized NC of CsA to a solid drug NP of CsA alone. These data have led to the finding that stabilizing block copolymer layers have a retarding effect on drug release from NCs, reducing the rate of CsA release fourfold compared with the nanoparticle without a polymer coating. This article is part of the themed issue 'Soft interfacial materials: from fundamentals to formulation'.

Original languageEnglish (US)
Article number20150128
JournalPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
Volume374
Issue number2072
DOIs
StatePublished - Jul 28 2016

All Science Journal Classification (ASJC) codes

  • Mathematics(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Keywords

  • Dissolution
  • Drug release
  • Magnetic nanoparticles
  • Nanocarriers
  • Nanoparticles

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