Detection of viral proteins using human receptor functionalized carbon nanotubes

M. Chen, S. M. Khamis, Sujit Sankar Datta, Y. B. Zhang, M. Kanungo, A. J. Ho, P. Freimuth, Daniel Van Der Lelie, A. T. Johnson, J. A. Misewich, S. S. Wong

Research output: Contribution to journalConference article

Abstract

We present proof-of-concept experiments for developing a highly-sensitive and fast- response miniaturized single-walled carbon nanotube field-effect transistor (SWNT-FET) biosensor for electrically detecting adenovirus using ligand-receptor-protein specificity. SWNTs are mildly oxidized to form carboxylic groups on the surfaces without compromising the electronic integrity of the nanotubes. Then the human coxsackievirus and adenovirus receptor (CAR) is covalently functionalized onto the nanotube surface via diimide-activated amidation process. Upon exposure of the device to adenovirus protein, Ad12 Knob (Knob), specific binding of Knob to CAR decreases the current that flows through the SWNT-FET device. For control experiment, the CAR-SWNT device is exposed to YieF, which is a virus protein that does not bind specifically to CAR, and no current change is observed. The biological activity of the CAR and Knob proteins that are immobilized on SWNTs has been confirmed by previous fluorescence studies [1]. AFM analysis is done to show height increase of a few nanometers at specific spots where the CAR-Knob complex are covalently linked to the nanotube surface. Therefore, our results show that the human receptor protein CAR does immobilize on SWNT surface while fully retains its biological activity. Moreover, the specific binding of CAR to its complementary adenovirus Knob can be electrically detected using individual SWNT-FET devices. These findings suggest that CAR-functionalized SWNT-FETs can ably serve as biosensors for detection of environmental adenoviruses.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalMaterials Research Society Symposium Proceedings
Volume1065
StatePublished - Dec 1 2008
Externally publishedYes
EventElectroactive and Conductive Polymers and Carbon Nanotubes for Biomedical Applications - Boston, MA, United States
Duration: Nov 26 2007Nov 30 2007

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Detection of viral proteins using human receptor functionalized carbon nanotubes'. Together they form a unique fingerprint.

  • Cite this

    Chen, M., Khamis, S. M., Datta, S. S., Zhang, Y. B., Kanungo, M., Ho, A. J., Freimuth, P., Lelie, D. V. D., Johnson, A. T., Misewich, J. A., & Wong, S. S. (2008). Detection of viral proteins using human receptor functionalized carbon nanotubes. Materials Research Society Symposium Proceedings, 1065, 15-20.