Abstract
We present a method for the analysis of optical single molecule emission data that exhibit discrete intensity jumps. This new method uses a generalized likelihood ratio test that determines the location of an intensity change point based on individual photon arrival times. This test is applied recursively to an entire single molecule intensity trajectory, thus finding each change points. Expectation-maximization clustering and the Bayesian information criterion is then used for accurate determination of the true number of states accessible to the system. This procedure allows rigorous and quantitative determination of intensity change points without the artificial time resolution limitations that arise from binning and thresholding.
Original language | English (US) |
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Pages (from-to) | 617-628 |
Number of pages | 12 |
Journal | Journal of Physical Chemistry B |
Volume | 109 |
Issue number | 1 |
DOIs | |
State | Published - Jan 13 2005 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Materials Chemistry
- Surfaces, Coatings and Films
- Physical and Theoretical Chemistry