Defective-interfering particles of Sindbis virus II. Homologous interference

Thomas E. Shenk; Victor Stollar

doi:10.1016/0042-6822(73)90197-9

Defective-interfering particles of Sindbis virus II. Homologous interference

Thomas E. Shenk
, Victor Stollar

Molecular Biology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A fraction containing defective-interfering particles of Sindbis virus was isolated by equilibrium density centrifugation of Sindbis virus stocks prepared by serial, undiluted passage through BHK-21 cells. This fraction had a mean density of 1.22 g/cc and interfered with the yield of Sindbis virus to a much greater extent than with Eastern equine encephalitis virus. Interfering activity was not limited to the 1.22 g/cc region, but extended through to the 1.20 g/cc fractions of the equilibrium density gradients.

Original language	English (US)
Pages (from-to)	530-534
Number of pages	5
Journal	Virology
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(73)90197-9
State	Published - Oct 1973

All Science Journal Classification (ASJC) codes

Virology

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10.1016/0042-6822(73)90197-9

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title = "Defective-interfering particles of Sindbis virus II. Homologous interference",

abstract = "A fraction containing defective-interfering particles of Sindbis virus was isolated by equilibrium density centrifugation of Sindbis virus stocks prepared by serial, undiluted passage through BHK-21 cells. This fraction had a mean density of 1.22 g/cc and interfered with the yield of Sindbis virus to a much greater extent than with Eastern equine encephalitis virus. Interfering activity was not limited to the 1.22 g/cc region, but extended through to the 1.20 g/cc fractions of the equilibrium density gradients.",

author = "Shenk, \{Thomas E.\} and Victor Stollar",

note = "Funding Information: We wish to thank Dr. R. Walter Schlesinger his comments and suggestions. This investigation was supported by the United States-Japan operative Medical Science Program administered by the National Institute Allergy and Infectious Diseases of National Institutes of Health Funding Information: T.E.S. was supported by a Na- of Health predoctoral fellowship The work reported in this paper was in the dissertation submitted by T.E.S. fulfillment of the requirements for the degree in Microbiology awarded by the School of Rutgers University. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1973",

month = oct,

doi = "10.1016/0042-6822(73)90197-9",

language = "English (US)",

volume = "55",

pages = "530--534",

journal = "Virology",

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T1 - Defective-interfering particles of Sindbis virus II. Homologous interference

AU - Shenk, Thomas E.

AU - Stollar, Victor

N1 - Funding Information: We wish to thank Dr. R. Walter Schlesinger his comments and suggestions. This investigation was supported by the United States-Japan operative Medical Science Program administered by the National Institute Allergy and Infectious Diseases of National Institutes of Health Funding Information: T.E.S. was supported by a Na- of Health predoctoral fellowship The work reported in this paper was in the dissertation submitted by T.E.S. fulfillment of the requirements for the degree in Microbiology awarded by the School of Rutgers University. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1973/10

Y1 - 1973/10

N2 - A fraction containing defective-interfering particles of Sindbis virus was isolated by equilibrium density centrifugation of Sindbis virus stocks prepared by serial, undiluted passage through BHK-21 cells. This fraction had a mean density of 1.22 g/cc and interfered with the yield of Sindbis virus to a much greater extent than with Eastern equine encephalitis virus. Interfering activity was not limited to the 1.22 g/cc region, but extended through to the 1.20 g/cc fractions of the equilibrium density gradients.

AB - A fraction containing defective-interfering particles of Sindbis virus was isolated by equilibrium density centrifugation of Sindbis virus stocks prepared by serial, undiluted passage through BHK-21 cells. This fraction had a mean density of 1.22 g/cc and interfered with the yield of Sindbis virus to a much greater extent than with Eastern equine encephalitis virus. Interfering activity was not limited to the 1.22 g/cc region, but extended through to the 1.20 g/cc fractions of the equilibrium density gradients.

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VL - 55

SP - 530

EP - 534

JO - Virology

JF - Virology

IS - 2

ER -

Defective-interfering particles of Sindbis virus II. Homologous interference

Abstract

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