Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo

Kristina Wehr, Andrew Swan, Trudi Schüpbach

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The deadlock gene is required for a number of key developmental events in Drosophila oogenesis. Females homozygous for mutations in the deadlock gene lay few eggs and those exhibit severe patterning defects along both the anterior-posterior and dorsal-ventral axis. In this study, we analyzed eggs and ovaries from deadlock mutants and determined that deadlock is required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females. Deadlock encodes a novel protein which colocalizes with the oocyte nucleus at midstages of oogenesis and with the centrosomes of early embryos. Our genetic and immunohistological experiments point to a role for Deadlock in microtubule function during oogenesis.

Original languageEnglish (US)
Pages (from-to)406-417
Number of pages12
JournalDevelopmental biology
Volume294
Issue number2
DOIs
StatePublished - Jun 15 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Keywords

  • Drosophila
  • Fusome
  • Germline stem cell
  • Microtubules
  • Oogenesis
  • Spermatogenesis

Fingerprint Dive into the research topics of 'Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo'. Together they form a unique fingerprint.

  • Cite this