Cytochemical observations of hemolymph cells during coagulation in the crayfish, orconectes virilis

Pauline J. Wood, John Podlewski, Thomas E. Shenk

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Smears of Orconectes virilis hemolymph were prepared by fixation in 10% formalin immediately upon withdrawal, and after an interval of 1, 5, or 15 minutes. These smears were then stained by a variety of histochemical means designed to identify a material which is released from both hyaline and granular cells during the coagulation process. Based upon reactions to various carbohydrate, lipid and protein tests, this material appears to consist of a glycoor muco‐protein, and its localization and activity coincide with the „fibrin ferment” described by Hardy (1892). Its release is probably initiated from the hyaline cells, followed shortly thereafter by a similar activity of the granulocytes. While it cannot be ascertained by the methods used in this study whether or not this material participates actively in clotting, its release is nevertheless coincidental with coagulation.

Original languageEnglish (US)
Pages (from-to)479-487
Number of pages9
JournalJournal of Morphology
Volume134
Issue number4
DOIs
StatePublished - Aug 1971
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Developmental Biology

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