TY - JOUR
T1 - Cytochemical observations of hemolymph cells during coagulation in the crayfish, orconectes virilis
AU - Wood, Pauline J.
AU - Podlewski, John
AU - Shenk, Thomas E.
PY - 1971/8
Y1 - 1971/8
N2 - Smears of Orconectes virilis hemolymph were prepared by fixation in 10% formalin immediately upon withdrawal, and after an interval of 1, 5, or 15 minutes. These smears were then stained by a variety of histochemical means designed to identify a material which is released from both hyaline and granular cells during the coagulation process. Based upon reactions to various carbohydrate, lipid and protein tests, this material appears to consist of a glycoor muco‐protein, and its localization and activity coincide with the „fibrin ferment” described by Hardy (1892). Its release is probably initiated from the hyaline cells, followed shortly thereafter by a similar activity of the granulocytes. While it cannot be ascertained by the methods used in this study whether or not this material participates actively in clotting, its release is nevertheless coincidental with coagulation.
AB - Smears of Orconectes virilis hemolymph were prepared by fixation in 10% formalin immediately upon withdrawal, and after an interval of 1, 5, or 15 minutes. These smears were then stained by a variety of histochemical means designed to identify a material which is released from both hyaline and granular cells during the coagulation process. Based upon reactions to various carbohydrate, lipid and protein tests, this material appears to consist of a glycoor muco‐protein, and its localization and activity coincide with the „fibrin ferment” described by Hardy (1892). Its release is probably initiated from the hyaline cells, followed shortly thereafter by a similar activity of the granulocytes. While it cannot be ascertained by the methods used in this study whether or not this material participates actively in clotting, its release is nevertheless coincidental with coagulation.
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U2 - 10.1002/jmor.1051340408
DO - 10.1002/jmor.1051340408
M3 - Article
C2 - 4106056
AN - SCOPUS:0015106108
SN - 0362-2525
VL - 134
SP - 479
EP - 487
JO - Journal of Morphology
JF - Journal of Morphology
IS - 4
ER -