Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

Toh Hean Ch'ng; E. Alexander Flood; Lynn William Enquist

Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

Toh Hean Ch'ng
, E. Alexander Flood
, Lynn William Enquist

Research output: Contribution to journal › Article › peer-review

Abstract

This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.

Original language	English (US)
Pages (from-to)	299-316
Number of pages	18
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	292
State	Published - 2005

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Cite this

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title = "Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.",

abstract = "This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.",

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AU - Ch'ng, Toh Hean

AU - Flood, E. Alexander

AU - Enquist, Lynn William

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AB - This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.

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UR - https://www.scopus.com/pages/publications/16644378729#tab=citedBy

M3 - Article

C2 - 15507717

AN - SCOPUS:16644378729

SN - 1064-3745

VL - 292

SP - 299

EP - 316

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

Abstract

All Science Journal Classification (ASJC) codes

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