This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.
|Original language||English (US)|
|Number of pages||18|
|Journal||Methods in molecular biology (Clifton, N.J.)|
|State||Published - Jan 1 2005|
All Science Journal Classification (ASJC) codes
- Molecular Biology