Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

Toh Hean Ch'ng, E. Alexander Flood, Lynn William Enquist

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.

Original languageEnglish (US)
Pages (from-to)299-316
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume292
StatePublished - Jan 1 2005

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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