CtBP-dependent activities of the short-range giant repressor in the Drosophila embryo

Yutaka Nibu, Michael S. Levine

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


There are at least three short-range gap repressors in the precellular Drosophila embryo: Krüppel, Knirps, and Giant. Krüppel and Knirps contain related repression motifs, PxDLSxH and PxDLSxK, respectively, which mediate interactions with the dCtBP corepressor protein. Here, we present evidence that Giant might also interact with dCtBP. The misexpression of Giant in ventral regions of transgenic embryos results in the selective repression of eve stripe 5. A stripe5-lacZ transgene exhibits an abnormal staining pattern in dCtBP mutants that is consistent with attenuated repression by Giant. The analysis of Gal4-Giant fusion proteins identified a minimal repression domain that contains a sequence motif, VLDLS, which is conserved in at least two other sequence-specific repressors. Removal of this sequence from the native Giant protein does not impair its repression activity in transgenic embryos. We propose that Giant-dCtBP interactions might be indirect and mediated by an unknown bZIP subunit that forms a heteromeric complex with Giant. We also suggest that the VLDLS motif recruits an as yet unidentified corepressor protein.

Original languageEnglish (US)
Pages (from-to)6204-6208
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number11
StatePublished - May 22 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'CtBP-dependent activities of the short-range giant repressor in the Drosophila embryo'. Together they form a unique fingerprint.

Cite this