Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton; Kevin Loutherback; David W. Inglis; James C. Sturm; Robert H. Austin; Stephen Y. Chou

Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton
, Kevin Loutherback
, David W. Inglis
, James C. Sturm
, Robert H. Austin
, Stephen Y. Chou

Research output: Contribution to conference › Paper › peer-review

Abstract

We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

Original language	English (US)
Pages	1965-1967
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Keywords

Cell lysis
Cell separation
Genomics
Sorting

Cite this

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title = "Crossing streamlines to lyse cells and simultaneously separate genomic contents",

abstract = "We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.",

keywords = "Cell lysis, Cell separation, Genomics, Sorting",

author = "Morton, \{Keith J.\} and Kevin Loutherback and Inglis, \{David W.\} and Sturm, \{James C.\} and Austin, \{Robert H.\} and Chou, \{Stephen Y.\}",

year = "2008",

language = "English (US)",

pages = "1965--1967",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Crossing streamlines to lyse cells and simultaneously separate genomic contents. / Morton, Keith J.; Loutherback, Kevin; Inglis, David W. et al.
2008. 1965-1967 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Crossing streamlines to lyse cells and simultaneously separate genomic contents

AU - Morton, Keith J.

AU - Loutherback, Kevin

AU - Inglis, David W.

AU - Sturm, James C.

AU - Austin, Robert H.

AU - Chou, Stephen Y.

PY - 2008

Y1 - 2008

N2 - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

AB - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

KW - Cell lysis

KW - Cell separation

KW - Genomics

KW - Sorting

UR - https://www.scopus.com/pages/publications/84902440707

UR - https://www.scopus.com/pages/publications/84902440707#tab=citedBy

M3 - Paper

AN - SCOPUS:84902440707

SP - 1965

EP - 1967

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Crossing streamlines to lyse cells and simultaneously separate genomic contents

Abstract

Other

All Science Journal Classification (ASJC) codes

Keywords

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