Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton, Kevin Loutherback, David W. Inglis, James Christopher Sturm, Robert Hamilton Austin, Stephen Y. Chou

Research output: Contribution to conferencePaperpeer-review

Abstract

We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

Original languageEnglish (US)
Pages1965-1967
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Keywords

  • Cell lysis
  • Cell separation
  • Genomics
  • Sorting

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