Crossing microfluidic streamlines to lyse, label and wash cells

Keith J. Morton, Kevin Loutherback, David W. Inglis, Ophelia K. Tsui, James C. Sturm, Stephen Y. Chou, Robert H. Austin

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

We present a versatile method for continuous-flow, on-chip biological processing of cells, large bio-particles, and functional beads. Using an asymmetric post array in pressure-driven microfluidic flow, we can move particles of interest across multiple, independent chemical streams, enabling sequential chemical operations. With this method, we demonstrate on-chip cell treatments such as labeling and washing, and bacterial lysis and chromosomal extraction. The washing capabilities of this method are particularly valuable because they allow many analytical or treatment procedures to be cascaded on a single device while still effectively isolating their reagents from cross-contamination.

Original languageEnglish (US)
Pages (from-to)1448-1453
Number of pages6
JournalLab on a Chip
Volume8
Issue number9
DOIs
StatePublished - 2008

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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