Crl facilitates RNA polymerase holoenzyme formation

Tamas Gaal, Mark J. Mandel, Thomas J. Silhavy, Richard L. Gourse

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The Escherichia coli Crl protein has been described as a transcriptional coactivator for the stationary-phase sigma factor σS. In a transcription system with highly purified components, we demonstrate that Crl affects transcription not only by the EσS RNA polymerase holoenzyme but also by Eσ70 and Eσ32. Crl increased transcription dramatically but only when the σ concentration was low and when Crl was added to σ prior to assembly with the core enzyme. Our results suggest that Crl facilitates holoenzyme formation, the first positive regulator identified with this mechanism of action.

Original languageEnglish (US)
Pages (from-to)7966-7970
Number of pages5
JournalJournal of bacteriology
Issue number22
StatePublished - Nov 2006

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


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