Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast

Kouichi Kuroda; Sarah K. Hammer; Yukio Watanabe; José Montaño López; Gerald R. Fink; Gregory Stephanopoulos; Mitsuyoshi Ueda; José L. Avalos

doi:10.1016/j.cels.2019.10.006

Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast

Kouichi Kuroda, Sarah K. Hammer, Yukio Watanabe, José Montaño López, Gerald R. Fink, Gregory Stephanopoulos, Mitsuyoshi Ueda, José L. Avalos

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

This study provides insights into the yeast response to isobutanol and mechanisms underlying specific toxicity and tolerance to isobutanol. Deletion of GLN3 markedly increases yeast tolerance specifically to branched-chain alcohols and isobutanol production. Deletion of GLN3 evades the native nitrogen starvation response induced by isobutanol, enhancing isobutanol tolerance. These findings illustrate how adaptive mechanisms to tolerate stress can lead to toxicity in chemical production and provide a promising strategy to boost production by genetically disrupting such mechanisms.

Original language	English (US)
Pages (from-to)	534-547.e5
Journal	Cell Systems
Volume	9
Issue number	6
DOIs	https://doi.org/10.1016/j.cels.2019.10.006
State	Published - Dec 18 2019

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology
Cell Biology

Keywords

GCN4
GLN3
Saccharomyces cerevisiae
TOR
alcohol tolerance
amino acid biosynthesis
genome-wide screen
isobutanol
nitrogen catabolite repression
pentose phosphate pathway

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10.1016/j.cels.2019.10.006

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title = "Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast",

abstract = "This study provides insights into the yeast response to isobutanol and mechanisms underlying specific toxicity and tolerance to isobutanol. Deletion of GLN3 markedly increases yeast tolerance specifically to branched-chain alcohols and isobutanol production. Deletion of GLN3 evades the native nitrogen starvation response induced by isobutanol, enhancing isobutanol tolerance. These findings illustrate how adaptive mechanisms to tolerate stress can lead to toxicity in chemical production and provide a promising strategy to boost production by genetically disrupting such mechanisms.",

keywords = "GCN4, GLN3, Saccharomyces cerevisiae, TOR, alcohol tolerance, amino acid biosynthesis, genome-wide screen, isobutanol, nitrogen catabolite repression, pentose phosphate pathway",

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doi = "10.1016/j.cels.2019.10.006",

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AU - Kuroda, Kouichi

AU - Hammer, Sarah K.

AU - Watanabe, Yukio

AU - Montaño López, José

AU - Fink, Gerald R.

AU - Stephanopoulos, Gregory

AU - Ueda, Mitsuyoshi

AU - Avalos, José L.

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Y1 - 2019/12/18

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KW - Saccharomyces cerevisiae

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KW - alcohol tolerance

KW - amino acid biosynthesis

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Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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