TY - JOUR
T1 - Cracking outer membrane biogenesis
AU - Guest, Randi L.
AU - Silhavy, Thomas J.
N1 - Funding Information:
We thank members of the Silhavy laboratory for their helpful discussions and feedback on the manuscript. Work in the Silhavy laboratory is funded by the National Institute of General Medical Sciences of the National Institutes of Health under award number 5R35GM118024 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2022
PY - 2023/2
Y1 - 2023/2
N2 - The outer membrane is a distinguishing feature of the Gram-negative envelope. It lies on the external face of the peptidoglycan sacculus and forms a robust permeability barrier that protects extracytoplasmic structures from environmental insults. Overcoming the barrier imposed by the outer membrane presents a significant hurdle towards developing novel antibiotics that are effective against Gram-negative bacteria. As the outer membrane is an essential component of the cell, proteins involved in its biogenesis are themselves promising antibiotic targets. Here, we summarize key findings that have built our understanding of the outer membrane. Foundational studies describing the discovery and composition of the outer membrane as well as the pathways involved in its construction are discussed.
AB - The outer membrane is a distinguishing feature of the Gram-negative envelope. It lies on the external face of the peptidoglycan sacculus and forms a robust permeability barrier that protects extracytoplasmic structures from environmental insults. Overcoming the barrier imposed by the outer membrane presents a significant hurdle towards developing novel antibiotics that are effective against Gram-negative bacteria. As the outer membrane is an essential component of the cell, proteins involved in its biogenesis are themselves promising antibiotic targets. Here, we summarize key findings that have built our understanding of the outer membrane. Foundational studies describing the discovery and composition of the outer membrane as well as the pathways involved in its construction are discussed.
KW - General secretion system
KW - Lipopolysaccharide transport
KW - Lipoprotein transport
KW - Outer membrane
KW - Phospholipid transport
KW - beta-barrel protein assembly machine
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U2 - 10.1016/j.bbamcr.2022.119405
DO - 10.1016/j.bbamcr.2022.119405
M3 - Article
C2 - 36455781
AN - SCOPUS:85143316063
SN - 0167-4889
VL - 1870
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 2
M1 - 119405
ER -