Cpx signaling pathway monitors biogenesis and affects assembly and expression of P pili

Danielle L. Hung, Tracy L. Raivio, C. Hal Jones, Thomas J. Silhavy, Scott J. Hultgren

Research output: Contribution to journalArticlepeer-review

151 Scopus citations


P pili are important virulence factors in uropathogenic Escherichia coli. The Cpx two-component signal transduction system controls a stress response and is activated by misfolded proteins in the periplasm. We have discovered new functions for the Cpx pathway, indicating that it may play a critical role in pathogenesis. P pili are assembled via the chaperone/usher pathway. Subunits that go "OFF-pathway" during pilus biogenesis generate a signal. This signal is derived from the misfolding and aggregation of subunits that failed to come into contact with the chaperone in the periplasm. In response, Cpx not only controls the stress response, but also controls genes necessary for pilus biogenesis, and is involved in regulating the phase variation of pap expression and, potentially, the expression of a panoply of other virulence factors. This study demonstrates how the prototypic chaperone/usher pathway is intricately linked and dependent upon a signal transduction system.

Original languageEnglish (US)
Pages (from-to)1508-1518
Number of pages11
JournalEMBO Journal
Issue number7
StatePublished - Apr 2 2001

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • General Neuroscience


  • Assembly
  • Cpx
  • P pili
  • Regulation


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